ICORP 2017, Rostock, 01. - 03.11.2017
Poster Sessions
| Author Name: | Selina Traxler - University Medicine of Rostock |
| Session Title: | |
| Event Type: | Oral Sessions |
| Event Title: | Volatile emmission of bacteria frequently involved in coinfections |
| Presider Name: | Co-Author: | Ann-Christin Bischoff, Gina Barkowsky, JK Schubert, Wolfram Miekisch | |
| Affiliation: | Affiliation: | University Medicine of Rostock |
| Date: | Thursday, 02.11.2017 | |
| Start Time: | 16:00 | |
| Location: |
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Abstract Content Bacterial and viral coinfections represent a crucial problem in clinical medicine. Volatile emissions may help to recognize organism growth and coinfections non-invasively. In order to understand these complex systems VOC profiles from typical coinfection agents such as Streptococcus pyogenes have to be investigated with respect to proliferation phases.
Method VOCs from headspaces above bacterial cultures were preconcentrated by means of needle trap devices (NTDs). Compounds were thermally desorbed from NTDs and analysed by GC-MS. VOC analysis was done on different time points after inoculation. Bacterial growth was assessed through determination of optical density of bacterial cultures. At every time point, headspace of three bacterial cultures was investigated. In parallel, three media samples were taken as control.
Results VOC profiles mirrored bacterial growth kinetics. Some VOCs were only detectable in the headspace of S. pyogenes and not in the medium. Several substances accumulated after 5 and 6 hours. After this maximum, concentrations of most substances decreased again. First accumulation of VOCs took place when growth of Streptococcus pyogenes changed from exponential to stationary growth phase.
Discussion Time course of VOC patterns mirrored active adjustments of metabolic pathways during bacterial proliferation. VOC analysis could be used for non-invasive monitoring of bacterial growth and could, therefore, indicate infection. VOC profiles from pure cultures could be compared with profiles from coinfections including S. pyogenes. Differences in emitted substances and their concentration could thus show changes and interactions during the process of coinfection. |
