Klinik und Poliklinik für Anästhesiologie und Intensivtherapie

2024

Jonas Pangerl, Pritam Sukul, Thomas Rück, Lukas Escher, Wolfram Miekisch, Rudolf Bierl, Frank-Michael Matysik. Photoacoustic Trace-Analysis of Breath Isoprene and Acetone via Interband- and Quantum Cascade Lasers, Sensors and Actuators B: Chemical, 2024, 136886, ISSN 0925-4005,
https://doi.org/10.1016/j.snb.2024.136886.

Wolfram Miekisch, Pritam Sukul, Jochen K. Schubert. Diagnostic potential of breath analysis – focus on the dynamics of volatile organic compounds, TrAC Trends in Analytical Chemistry, 2024, https://doi.org/10.1016/j.trac.2024.117977

Jonas Pangerl, Pritam Sukul, Thomas Rück, Patricia Fuchs, Stefan Weigl, WolframMiekisch, Rudolf Bierl, Frank-Michael Matysik. An inexpensive UV-LED photo-acoustic based real-time sensor-system detecting exhaled trace-acetone. Photoacoustics. 38 (2024) 100604 https://doi.org/10.1016/j.pacs.2024.100604

Kemnitz N, Fuchs P, Remy R, Ruehrmund L, Bartels J, Klemenz AC, Trefz P, Miekisch W, Schubert JK, Sukul P. Effects of Contagious Respiratory Pathogens on Breath Biomarkers. Antioxidants (Basel). 2024 Jan 29;13(2):172. https://doi.org/10.3390/antiox13020172

Leo Ruehrmund, PatriciaFuchs, Julia Bartels, Rasmus Remy, Ann-Christin Klemenz, Nele Kemnitz, Phillip Trefz, Pritam Sukul, Wolfram Miekisch, Jochen K.Schubert. Protocol for visualizing complex volatile metabolomics data in clinical setups using EDaViS software. STAR Protocols, 2024 Jan 1;5(1):102808.doi:10.1016/j.xpro.2023.102808 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10797205/

2023

Vanessa Wolfschluckner, Beate Obermüller, Angela Horvath, Giovanny Rodriguez-Blanco, Patricia Fuchs, Wolfram Miekisch, Barbara Mittl, Christina Flucher, Holger Till, Georg Singer. Metabolomic Alterations of Volatile Organic Compounds and Bile Acids as Biomarkers of Microbial Shifts in a Murine Model of Short Bowel Syndrome. Nutrients 2023 Nov 29;15(23):4949. doi: 10.3390/nu15234949. https://www.mdpi.com/2072-6643/15/23/4949

Obermüller B, Singer G, Kienesberger B, Mittl B, Stadlbauer V, Horvath A, Miekisch W, Fuchs P, Schweiger M, Pajed L, Till H, Castellani C. Probiotic OMNi-BiOTiC^® 10 AAD Reduces Cyclophosphamide-Induced Inflammation and Adipose Tissue Wasting in Mice. Nutrients. 2023 Aug 20;15(16):3655. doi: 10.3390/nu15163655. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458463/

Pritam Sukul, Anna Richter, Christian Junghanss, Jochen K Schubert, Wolfram Miekisch. Origin of breath isoprene in humans is revealed via multi-omic investigations. Communications Biology (2023)6:999 https://doi.org/10.1038/s42003-023-05384-y

Kai Kisielinski, Oliver Hirsch, Susanne Wagner, Barbara Wojtasik, Stefan Funken, Bernd Klosterhalfen, Soumen Kanti Manna, Andreas Prescher, Pritam Sukul and Andreas Sönnichsen. Physio-metabolic and clinical consequences of wearing face masks -Systematic review with meta-analysis and comprehensive evaluation. Front. Public Health (2023) 11:1125150 https://www.frontiersin.org/articles/10.3389/fpubh.2023.1125150/full

2022

Pritam Sukul, Phillip Trefz, Jochen K Schubert, Wolfram Miekisch. Advanced setup for safe breath sampling and patient monitoring under highly infectious conditions in the clinical environment. Sci Rep 12, 17926 (2022).https://doi.org/10.1038/s41598-022-22581-7

Remy R, Kemnitz N, Trefz P, Fuchs P, Bartels J, Klemenz AC, Rührmund L, Sukul P, Miekisch W, Schubert JK. Profiling of exhaled volatile organics in the screening scenario of a COVID-19 test center. iScience. 2022 Oct 21;25(10):105195. doi:10.1016/j.isci.2022.105195. Epub 2022 Sep 23.

Miekisch W, Sukul P, Schubert J K. Chapter 2: Origin and Emission of Volatile Biomarkers in Breath: Basics and Dynamic Aspects. In: Volatile Biomarkers for Human Health: From Nature to Artificial Senses. Ed. Haick H. The Royal Society of Chemistry 2022 https://doi.org/10.1039/9781839166990-00022

Sukul, P., Trefz, P. (2022). Physio-Metabolic Monitoring via Breath Employing Real-Time Mass Spectrometry: Importance, Challenges, Potentials, and Pitfalls. In: Bioanalytical Reviews. Springer, Berlin, Heidelberg. doi.org/10.1007/11663_2022_19

Giovanni Pugliese, Phillip Trefz, Matthias Weippert, Johannes Pollex, Sven Bruhn, Jochen K Schubert, Wolfram Miekisch, Pritam Sukul. Real-time metabolic monitoring under exhaustive exercise and evaluation of ventilatory threshold by breathomics: independent validation of evidence and advances. Front. Physiol., 12 August 2022 Sec. Exercise Physiology. doi.org/10.3389/fphys.2022.946401

Beate Brock, Patricia Fuchs, Svend Kamysek, Udo Walther, Selina Traxler, Giovanni Pugliese, Wolfram Miekisch, Jochen K. Schubert, Phillip Trefz. Non-invasive o-toluidine monitoring during regional anaesthesia with prilocaine and detection of accidental intravenous injection in an animal model. Metabolites 2022 May 31;12(6):502. doi: 10.3390/metabo12060502.

Kienesberger, B.; Obermüller, B.; Singer, G.; Arneitz, C.; Gasparella, P.; Klymiuk, I.; Horvath, A.; Stadlbauer, V.; Magnes, C.; Zügner, E.; López-García, P.; Trajanoski, S.; Miekisch, W.; Fuchs, P.; Till, H.; Castellani, C. Insights into the composition of a co-culture of 10 probiotic strains (OMNi BiOTiC® AAD10) and effects of its postbiotic culture supernatant. Nutrients 2022 Mar 11;14(6):1194. doi: 10.3390/nu14061194

Sukul P, Bartels J, Fuchs P, Trefz P, Remy R, Rührmund L, Kamysek S, Schubert JK, Miekisch W. Effects of COVID-19 protective face-masks and wearing durations onto respiratory-haemodynamic physiology and exhaled breath constituents. European Respiratory Journal 2022 60 (3) 2200009 https://doi.org/10.1183/13993003.00009-2022

Pritam Sukul, Simon Grzegorzewsk, Celine Broderius, Phillip Trefz, Thomas Mittlmeier, Dagmar-Christiane Fischer, Wolfram Miekisch, Jochen K Schubert.^.Physiological and metabolic effects of healthy female aging on exhaled breath biomarkers. iScience 25,103739 Febuary 18, 2022. doi: 10.1016/j.isci.2022.103739

Wang N, Pugliese G, Carrito M, Moura C, Vasconcelos P, Cera N, Li M, Nobre P, Georgiadis JR, Schubert JK, Williams J. Breath chemical markers of sexual arousal in humans. Sci Rep. 2022 Apr 15;12(1):6267. doi: 10.1038/s41598-022-10325-6.

2021

Henderson B, Ruszkiewicz DM, Wilkinson M, Beauchamp JD, Cristescu SM, Fowler SJ, Salman D, Francesco FD, Koppen G, Langejürgen J, Holz O, Hadjithekli A, Moreno S, Pedrotti M, Sinues P, Slingers G, Wilde M, Lomonaco T, Zanella D, Zenobi R, Focant JF, Grassin-Delyle S, Franchina FA, Malásková M, Stefanuto PH, Pugliese G, Mayhew C, Thomas CLP. A benchmarking protocol for breath analysis: the peppermint experiment. J Breath Res. 2020 Aug 10;14(4):046008. doi: 10.1088/1752-7163/aba130.

Castellani C, Obermüller B, Kienesberger B, Singer G, Peterbauer C, Grabherr R, Mayrhofer S, Klymiuk I, Horvath A, Stadlbauer V, Russmayer H, Miekisch W, Fuchs P, Till H, Heinl S. Production, Storage Stability, and Susceptibility Testing of Reuterin and Its Impact on the Murine Fecal Microbiome and Volatile Organic Compound Profile. Front Microbiol. 2021 Jul 30;12:699858. doi: 10.3389/fmicb.2021.699858.

Kienesberger B, Obermüller B, Singer G, Mittl B, Grabherr R, Mayrhofer S, Heinl S, Stadlbauer V, Horvath A, Miekisch W, Fuchs P, Klymiuk I, Till H, Castellani C. (S)-Reutericyclin: Susceptibility Testing and In Vivo Effect on Murine Fecal Microbiome and Volatile Organic Compounds. Int J Mol Sci. 2021 Jun 15;22(12):6424. doi: 10.3390/ijms22126424.

Warncke G, Singer G, Windhaber J, Schabl L, Friehs E, Miekisch W, Gierschner P, Klymiuk I, Eber E, Zeder K, Pfleger A, Obermüller B, Till H, Castellani C. Volatile Organic Compounds, Bacterial Airway Microbiome, Spirometry and Exercise Performance of Patients after Surgical Repair of Congenital Diaphragmatic Hernia. Molecules. 2021 Jan 26;26(3):645. doi: 10.3390/molecules26030645.

Vitense P, Kasbohm E, Klassen A, Gierschner P, Trefz P, Weber M, Miekisch W, Schubert JK, Möbius P, Reinhold P, Liebscher V, Köhler H. Detection of Mycobacterium avium ssp. paratuberculosis in Cultures From Fecal and Tissue Samples Using VOC Analysis and Machine Learning Tools. Front Vet Sci. 2021 Feb 3;8:620327. doi: 10.3389/fvets.2021.620327.

Sukul P, Richter A, Schubert JK, Miekisch W. Deficiency and absence of endogenous isoprene in adults, disqualified its putative origin. Heliyon. 2021 Jan 12;7(1):e05922. doi: 10.1016/j.heliyon.2021.e05922.

2020

Fuchs, Patricia; Trautner, Markus; Sass, Radost; Kamysek, Svend; Miekisch, Wolfram; Bier, Andrea; Stoll, Paul; Schubert, Jochen. Spatial mapping of VOC exhalation by means of bronchoscopic sampling. Journal of Breath Research 2020 https://doi.org/10.1088/1752-7163/abb478

Giovanni Pugliese, Felix Piel, Phillip Trefz, Philipp Sulzer, Jochen K. Schubert, Wolfram Miekisch. Effects of modular ion-funnel technology onto analysis of breath VOCs by means of real-time mass spectrometry. Analytical and Bioanalytical Chemistry (2020) 412:7131–7140

Jorge A. M. Pereira, Priscilla Porto-Figueira, Ravindra Taware, Pritam Sukul, Srikanth Rapole and José S. Câmara. Unravelling the Potential of Salivary Volatile Metabolites in Oral Diseases. A Review. Molecules 2020, 25, 3098; doi:10.3390/molecules25133098

Pritam Sukul, Jochen Schubert, Karim Zanaty, Phillip Trefz, Anupam Sinha, Svend Kamysek, Wolfram Miekisch. Exhaled breath compositions under varying respiratory rhythms reflects ventilatory variations: translating breathomics towards respiratory medicine. Sci Rep. 2020 Aug 24;10(1):14109. doi: 10.1038/s41598-020-70993-0.

Benjamin Löser, Alina Grabenschröer, Giovanni Pugliese, Pritam Sukul, Phillip Trefz , Jochen K Schubert and Wolfram Miekisch. Changes of Exhaled Volatile Organic Compounds in Postoperative Patients Undergoing Analgesic Treatment: A Prospective observational Study. Metabolites 2020, 10, 321; Doi:10.3390/metabo10080321

Beate Obermueller, Georg Singer , Bernhard Kienesberger, Ingeborg Klymiuk, Daniela Sperl, Vanessa Stadlbauer, Angela Horvath, Wolfram Miekisch, Peter Gierschner, Reingard Grabherr, Hans-Juergen Gruber, Maria D Semeraro, Holger Till, Christoph Castellani The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds and Gut Permeability in Murine Neuroblastoma Induced Tumor Associated Cachexia. Nutrients 2020 Jul 8;12(7):2029. doi: 10.3390/nu12072029 PMID 32650568

Schubert JK, Miekisch W. Breath Monitoring in the Intensive Care Unit, in Breathborne Biomarkers and the Human Volatilome, 2nd Edition. Ed. Beauchamp J, Davis C, Pleil J. Amsterdam, 2020 p 289-304

Beauchamp J, Miekisch W. Breath Sampling and Standardization, in Breathborne Biomarkers and the Human Volatilome, 2nd Edition. Ed. Beauchamp J, Davis C, Pleil J., Amsterdam 2020, p 23-42

Petra E. Reinhold, Peter Gierschner, Anne Küntzel, Jochen K. Schubert, Wolfram Miekisch and Heike U. Köhler. Ruminants, in Breathborne Biomarkers and the Human Volatilome, 2nd Edition. Ed. Beauchamp J, Davis C, Pleil J., Amsterdam 2020, p 441-460

2019

Selina Traxler, Gina Barkowsky, Radost Saß, Ann-Christin Klemenz, Nadja Patenge, Bernd Kreikemeyer, Jochen K Schubert and Wolfram Miekisch. Volatile scents of influenza A and S. pyogenes (co-)infected cells. Scientific Reports (2019) 9:18894 | https://doi.org/10.1038/s41598-019-55334-0

Phillip Trefz , Sibylle Schmidt, Pritam Sukul, Jochen Schubert, Wolfram Miekisch, Dagmar-Christiane Fischer. Non-invasive assessment of metabolic adaptation in paediatric patients suffering from type 1 diabetes mellitus. Journal of Clinical Medicine 2019, in press

Giovanni Pugliese, Phillip Trefz, Beate Brock, Jochen K. Schubert, Wolfram Miekisch. Extending PTR based breath analysis to real-time monitoring of reactive volatile organic compounds. Analyst 2019, 144, 7359 – 7367

Phillip Trefz, Juliane Obermeier, Ruth Lehbrink, Jochen K Schubert, Wolfram Miekisch and Dagmar-Christiane Fischer. Exhaled volatile substances in children suffering from type 1 diabetes mellitus: results from a cross-sectional study. Scientific Reports 2019 in press.

Küntzel A, Weber M, Gierschner P, Trefz P, Miekisch W, Schubert JK, Reinhold P, Köhler H. Core profile of volatile organic compounds related to growth of Mycobacterium avium subspecies paratuberculosis - A comparative extract of three independent studies. PLoS One. 2019 Aug 15;14(8):e0221031. doi: 10.1371/journal.pone.0221031. eCollection 2019.

Klemenz AC, Meyer J, Ekat K, Bartels J, Traxler S, Schubert JK, Kamp G, Miekisch W and Peters K. Differences in the Emission of Volatile Organic Compounds (VOCs) between Non-Differentiating and Adipogenically Differentiating Mesenchymal Stromal/Stem Cells from Human Adipose Tissue. Cells 2019, 8(7), 697; doi: 10.3390/cells8070697.

Trefz P, Pugliese G, Brock B, Schubert JK, Miekisch W. Effects of elevated oxygen levels on VOC analysis by means of PTR-ToF-MS. J Breath Res. 2019 Jul 9;13(4):046004. doi: 10.1088/1752-7163/ab28ec.

Pleil JD, Wallace A, Di Francesco F, Miekisch W. Contemporary human breath related topics: aerosols, saliva, and HR-MS bioinformatics from Pittcon 2019. J Breath Res. 2019 May 7. doi: 10.1088/1752-7163/ab1fa8.

Gierschner P, Küntzel A, Reinhold P, Köhler H, Schubert J, Miekisch W. Crowd monitoring in dairy cattle – Real-time VOC profiling by direct mass spectrometry. J Breath Res, 2019 Jul 11;13(4):046006. doi: 10.1088/1752-7163/ab269f.

Castellani C, Singer G, Eibisberger M, Obermüller B, Warncke G, Miekisch W, Kolb-Lenz D, Summer G, Pauer TM, ElHaddad A, Kashofer K, Till H. The effects of neuroblastoma and chemoterapy on metabolism, fecal microbiome, volatile organic compounds, and gut barrier function in a murine model. Pediatr Res. 2019 Jan. doi: 10.1038/s41390-019-0283-1.

2018

Trefz P, Schubert JK, Miekisch W. Effects of humidity, CO₂ and O₂ on real-time quantitation of breath biomarkers by means of PTR-ToF-MS. J Breath Res. 2018 Mar 1;12(2):026016. doi: 10.1088/1752-7163/aa9eea.

Püschel A, Ebel R, Fuchs P, Hofmann J, Schubert JK, Roesner JP, Bergt S, Wree A, Vollmar B, Klar E, Bünger CM, Kischkel S. Can Recognition of Spinal Ischemia Be Improved? Application of Motor-Evoked Potentials, Serum Markers, and Breath Gas Analysis in an Acutely Instrumented Pig Model. Ann Vasc Surg. 2018 Mar 5. pii: S0890-5096(18)30220-6. doi: 10.1016/j.avsg.2018.01.095.

Oertel P, Küntzel A, Reinhold P, Köhler H, Schubert J, Kolb J, Miekisch W. Continuous real-time breath analysis in ruminants: Effect of eructation on exhaled VOC profiles. J Breath Res. 2018 Apr 12. doi: 10.1088/1752-7163/aabdaf.

Küntzel A, Oertel P, Fischer S, Bergmann A, Trefz P, Schubert J, Miekisch W, Reinhold P, Köhler H. Comparative analysis of volatile organic compounds for the classification and identification of mycobacterial species. PLoS One. 2018 Mar 20;13(3):e0194348. doi: 10.1371/journal.pone.0194348.

Bischoff AC, Oertel P, Sukul P, Rimmbach C, David R, Schubert J, Miekisch W. Smell of cells: Volatile profiling of stem- and non-stem cell proliferation. J Breath Res. 2018 Mar 1;12(2):026014. doi: 10.1088/1752-7163/aaa111

Traxler S, Bischoff AC, Trefz P, Schubert J and Miekisch W. Versatile set-up for non-invasive in vitro analysis of headspace VOCs. J Breath Res. 2018 June. doi: 10.1088/1752-7163/aaccad.

Sukul P, Schubert JK, Trefz P and Miekisch W. Natural Menstrual rhythm and oral exhaled breath compositions. Scientific Reports. 2018 Jul doi:10.1038/s41598.018.29221.z.

Porto-Figueira P, Pereira J, Miekisch W, Câmara JS. Exploring the potential of NTME/GC-MS, in the establishment of urinary volatomic profiles. Lung cancer patients as case study. Sci Rep. 2018 Aug 30;8(1):13113. doi: 10.1038/s41598-018-31380-y

Traxler S, Bischoff AC, Saß R, Trefz P, Gierschner P, Brock B, Schwaiger T, Karte C, Blohm U, Schröder C, Miekisch W, Schubert JK. VOC breath profile in spontaneously breathing awake swine during Influenza A infection. Scientific Reports volume 8, Article number: 14857 (2018). doi:10.1038/s41598-018-33061-2

2017

Brock B, Kamysek S, Silz J, Trefz P, Schubert JK, Miekisch W. Monitoring of breath VOCs and electrical impedance tomography under pulmonary recruitment in mechanically ventilated patients. J. Breath Res. 11 (2017) 016005

Timm U, Gewiss H, Kraitl J, Brock B, Ewald H. Sensor system for non-invasive optical carboxy- and methemoglobin determination. Optical Diagnostics and Sensing XVI: Toward Point-of-Care-Diagnostics, Proc. SPIE, 2017. 10072, 100720Y-1. DOI: 10.1117/12.2252549.

Kischkel S, Bergt S, Brock B, von Grönheim J, Herbst A, Epping MJ, Matheis G, Novosel E, Schneider J, Warnke P, Podbielski A, Roesner JP, Lelkes PI, Vollmar B. In-vivo testing of extracorporeal membrane ventilators: ILA activve vs. prototype I-lung. ASAIO J. 2017 Mar/Apr;63(2):185-192. DOI: 10.1097/MAT.0000000000000465.

Trefz P, Kamysek S, Fuchs P, Sukul P, Schubert JK, Miekisch W. Drug detection in breath: non-invasive assessment of illicit or pharmaceutical drugs. J. Breath Res. 11 (2017) 024001

Sukul P, Oertel P, Kamysek S and Trefz P. Oral or nasal breathing? Real-time effects of switching sampling route onto exhaled VOC concentrations. J. Breath Res. 11 (2017), Number 2

Gewiß H, Timm U, Kraitl J, Brock B and Ewald H. Non-invasive multi wavelengths sensorsystem for measuring carboxy - and methemoglobin. Current Directions in Biomedical Engineering 2017; 3(2):441-444. DOI: 10.1515/cdbme-2017-0092.

Obermeier J, Trefz P, Happ J, Staude H, Schubert J K, Fischer D-C, Miekisch W. Exhaled volatile substances mirror clinical conditions in pediatric chronic kidney disease. PLoS One. 2017 Jun 1;12(6):e0178745.

Kasbohm, E; Fischer, S; Küntzel, A; Oertel, P; Bergmann, A; Trefz, P; Miekisch, W; Schubert, JK; Reinhold, P; Ziller, M; Fröhlich, A; Liebscher, V; Köhler, H. Strategies for the identification of disease-related patterns of volatile organic compounds: Prediction of paratuberculosis in an animal model using random forests. J Breath Res. 2017 Aug 3. doi: 10.1088/1752-7163/aa83bb.

Sukul, P; Schubert, JK; Kamysek, S; Trefz, P; Miekisch, W. Applied upper-airway resistance instantly affects breath components: a unique insight into pulmonary medicine. Article reference: JBR-100652.R2

Brock B, Kamysek S, Trefz P, Fuchs P, Timm U, Miekisch W and Schubert JK. O-Toluidine in Breath-Non-Invasive Prilocaine Monitoring. J of Clin Exp Pharmacol 2017; 7:242. DOI: 10.4172/2161-1459.1000242.

Fuchs P, Obermeier J, Kamysek S, Degner M, Nierath H, Juerss H, Ewald H, Schwarz J, Becker M, Schubert JK. Safety and applicability of a pre-stage public access ventilator for trained laypersons: a proof of principle study. BMC Emergency Medicine, 2017. DOI 10.1186/s12873-017-0150-5

2016

Sukul P, Schubert JK, Oertel P, Kamysek S, Taunk K, Trefz P, Miekisch W. FEV manoeuvre induced changes in breath VOC compositions: an unconventional view on lung function tests. Scientific Reports 6, Article number: 28029 (2016) doi:10.1038/srep28029

Trepesch C, Nitzsche R, Glass A, Kreikemeyer B, Schubert JK, Oehmcke-Hecht S. High intravascular tissue factor—but not extracellular microvesicles—in septic patients is associated with a high SAPS II score.Journal of Intensive Care (2016) 4:34 DOI 10.1186/s40560-016-0160-5

Küntzel A, Fischer S, Bergmann A, Oertel P, Steffens M, Trefz P, Miekisch W, Schubert JK, Reinhold P, Köhler H. Effects of biological and methodological factors on volatile organic compound patterns during cultural growth of Mycobacterium avium ssp. paratuberculosis. J Breath Res. 2016 Sep 8;10(3):037103. doi: 10.1088/1752-7155/10/3/037103.

2015

Fischer S, Bergmann A, Steffens M, Trefz P, Ziller M, Miekisch W, Schubert JS, Köhler H, Reinhold P.Impact of food intake on in vivo VOC concentrations in exhaled breath assessed in a caprine animal model. J Breath Res. 2015 Dec 15;9(4):047113. doi: 10.1088/1752-7155/9/4/047113.

Sukul P, Trefz P, Kamysek S, Schubert JK, Miekisch W. Instant effects of changing body positions on compositions of exhaled breath. J Breath Res. 2015 Nov 19;9(4):047105. doi: 10.1088/1752-7155/9/4/047105.

Pleil JD, Beauchamp JD, Miekisch W, Funk WE.Adapting biomarker technologiesto adverse outcome pathways (AOPs) research: current thoughts on using in vivodiscovery for developing in vitro target methods.J Breath Res. 2015 Jul 14;9(3):039001. doi: 10.1088/1752-7155/9/3/039001.

Fischer S,Trefz P, Bergmann A, Steffens M,Ziller M,Miekisch W, Schubert JS,Köhler H, Reinhold P.Physiological variability in volatile organic compounds (VOCs) in exhaled breath and released from faeces due to nutrition and somatic growth in a standardized caprine animal model. J Breath Res. 2015 May 14;9(2):027108. doi: 10.1088/1752-7155/9/2/027108.

Bergmann A, Trefz P, Fischer S,Klepik K,Walter G,Steffens M,Ziller M, Schubert JK, Reinhold P, Köhler H,Miekisch W.In Vivo Volatile Organic Compound Signatures of Mycobacterium avium subsp. paratuberculosis. PLoS One. 2015 Apr 27;10(4):e0123980. doi: 10.1371/journal.pone.0123980. eCollection 2015.

Obermeier J, Trefz P,Wex K,Sabel B, Schubert JK, Miekisch W.Electrochemical sensor system for breath analysis of aldehydes, CO and NO. J Breath Res. 2015 Mar 9;9(1):016008. doi: 10.1088/1752-7155/9/1/016008.

2014

Sukul P, Trefz P, Schubert JK, Miekisch W. Immediate effects of breath holding maneuvers onto composition of exhaled breath. J Breath Res. 2014 Sep;8(3):037102. doi: 10.1088/1752-7155/8/3/037102.

Miekisch W, Trefz P, Bergmann A, Schubert JK. Microextraction techniques in breath biomarker analysis. Bioanalysis. 2014 May;6(9):1275-91. Review.

Amann A, Miekisch W, Schubert J, Buszewski B, Ligor T, Jezierski T, Pleil J, Risby T. Analysis of exhaled breath for disease detection. Annu Rev Anal Chem (Palo Alto Calif). 2014;7:455-82. doi: 10.1146/annurev-anchem-071213-020043.

Amann A, Costello Bde L, Miekisch W, Schubert J, Buszewski B, Pleil J, Ratcliffe N, Risby T. The human volatilome: volatile organic compounds (VOCs) in exhaled breath, skin emanations, urine, feces and saliva. J Breath Res. 2014 Sep;8(3):034001. doi: 10.1088/1752-7155/8/3/034001.

Pleil JD, Miekisch W, Stiegel MA, Beauchamp J. Extending breath analysis to the cellular level: current thoughts on the human microbiome and the expression of organic compounds in the human exposome. J Breath Res. 2014 Jun;8(2):029001. doi: 10.1088/1752-7155/8/2/029001.

2013

Anthony Alozie, Bernd Westphal, Stephan Kische, Alexander Kaminski, Liliya Paranskaya, Ilkay Bozdag-Turan, Jasmin Ortak, Jochen Schubert, Gustav Steinhoff, Hüseyin Ince. Surgical revision after percutaneous mitral valve repair by edge-to-edge device: when the strategy fails in the highest risk surgical population.European journal of cardio-thoracic surgery: official journal of the European Association for Cardio-thoracic Surgery (Impact Factor: 2.4). 12/2013; DOI:10.1093/ejcts/ezt53

Kleeblatt J, Ehlert S, Hölzer J, Sklorz M, Rittgen J, Baumgärtel P, Schubert JK, Zimmermann R. Investigation of the photoionization properties of pharmaceutically relevant substances by resonance-enhanced multiphoton ionization spectroscopy and single-photon ionization spectroscopy using synchrotron radiation. Appl Spectrosc. 2013 Aug;67(8):860-72. doi: 10.1366/13-06988

Trefz P, Schmidt M, Oertel P, Obermeier J, Brock B, Kamysek S, Dunkl J, Zimmermann R, Schubert JK, Miekisch W. Continuous real time breath gas monitoring in the clinical environment by proton-transfer-reaction-time of flight-mass spectrometry. Anal Chem. 2013 Nov 5;85(21):10321-9.

Trefz P, Koehler H, Klepik K, Moebius P, Schubert J, Reinhold P, Miekisch W. Volatile emissions from Mycobacterium avium subsp. paratuberculosis mirror bacterial growth and enable distinction of different strains. PLoS One. 2013 Oct 8;8(10):e76868. doi: 10.1371/journal.pone.0076868

Trefz P, Rösner L, Hein D, Schubert JK, Miekisch W. Evaluation of needle trap micro-extraction and automatic alveolar sampling for point-of-care breath analysis. Anal Bioanal Chem. 2013 Apr;405(10):3105-15.

Goerl T, Kischkel S, Sawacki A, Fuchs P, Miekisch W, Schubert JK. Volatile breath biomarkers for patient monitoring during haemodialysis. J Breath Res. 2013 Mar;7(1):017116. doi: 10.1088/1752-7155/7/1/017116. Epub 2013 Feb 27

2012 und davor

Miekisch W, Herbig, Schubert JK. Data interpretation in breath biomarker research: pitfalls and directions.J. Breath Res. 6 (2012) 036007 (10pp); doi:10.1088/1752-7155/6/3/036007

Paranskaya L, D'Ancona G, Bozdag-Turan I, Akin I, Kische S, Turan GR, Divchev D, Rehders T, Westphal B, Schubert J, Nienaber CA, Ince H. Percutaneous mitral valve repair with the MitraClip® system: Perioperative and 1-year follow-up results using standard or multiple clipping strategy. Catheter Cardiovasc Interv. 2012 Jun 28. doi: 10.1002/ccd.24538. [Epub ahead of print]

Schubert R, Schwoebel H, Mau-Moeller A, Behrens M, Fuchs P, Sklorz M, Schubert JK, Bruhn S, Miekisch W. Metabolic monitoring and assessment of anaerobic threshold by means of breath biomarkers.Metabolomics 2012; 8. 1069-1080.

Kischkel S, Miekisch W, Fuchs P, Schubert JK. Breath analysis during one-lung ventilation in cancer patients. Eur Respir J 2012. doi: 10.1183/09031936.00125411.

Hergert SM, Koball S, Machmüller S, Nöldge-Schomburg GEF, Schubert JK, Roesner JP. Vancomycintherapie und Niereninsuffizienz – eine verhängnisvolle Kombination und Ihre Behandlung (Vancomycin treatment and renal failure – a dramatic situation and its management). Anästh Intensivmed 2012;1:46-49.

Trefz P, Kischkel S, Hein D, James ES, Schubert JK, Miekisch W. Needle trap micro-extraction for VOC analysis: effects of packing materials and desorption parameters. J Chromatography A. 2011; 1219:29-38.

Kamysek S, Fuchs P, Schwoebel H, Roesner JP, Kischkel S, Wolter K, Loeseken C, Schubert JK, Miekisch W. Drug detection in breath: Effects of pulmonary blood flow and cardiac output on propofol exhalation.Anal Bioanal Chem. 2011;401(7):2093-102.

Schwöbel HU, Schubert R, Sklorz M, Kischkel S, Zimmermann R, Schubert JK, Miekisch W. Phase resolved real-time breath analysis during exercise by means of smart processing of PTR-MS data. Anal Bioanal Chem. 2011; 401(7):2079-91.

Sabel B, Schubert J, Miekisch W, Fuchs P, Kischkel S, Mieth M. Comparison of different preconcentration techniques in breath analysis under real life clinical conditions. Eur J Med Res 2010; 15:1-1.

Sponring A, Filipiak W, Ager C, Schubert J, Miekisch W, Amann A, Troppmair J. Analysis of volatile organic compounds (VOCs) in the headspace of NCI-H1666 lung cancer cells. Cancer Biomark. 2010;7(3):153-61.

Amann A, Ligor M, Ligor T, Bajtarevic A, Ager C, Pienz M, Denz H, Fiegl M, Hilbe W, Weiss W, Lukas P, Jamnig H, Hackl M, Haidenberger A, Sponring A, Filipiak W, Miekisch W, Schubert J, Troppmair J, Buszewski B. Analysis of exhaled breath for screening of lung cancer patients. Memo 2010; 3:106-112.

Olszowy P, Szultka M, Fuchs P, Kegler R, Mundkowski R, Miekisch W, Schubert JK, Boguslaw Buszewski B. New coated SPME fibers for extraction and fast HPLC determination of selected drugs in human blood. J Pharm Biomed Anal. 2010; 53(4):1022-7.

Kischkel S, Miekisch W, Sawacki A, Straker EM, Trefz P, Amann A, Schubert JK. Breath biomarkers for lung cancer detection and assessment of smoking related effects - confounding variables, influence of normalization and statistical algorithms. Clin Chim Acta. 2010; 411(21-22):1637-44.

Szultka M, Kegler R, Fuchs P, Olszowy P; Miekisch W, Schubert JK, Buszewski B, Mundkowski RG. Polypyrrole solid phase microextraction: a new approach to rapid sample preparation for the monitoring of antibiotic drugs. Anal Chim Acta. 2010; 667(1-2):77-82.

Fuchs P, Loeseken C, Schubert JK, Miekisch W. Breath gas aldehydes as biomarkers of lung cancer. Int J Cancer. 2010; 126(11):2663–2670.

Mieth M, Schubert J, Gröger T, Sabel B, Kischkel S, Fuchs P, Hein D, Zimmermann R, Miekisch M. Automated Needle Trap Heartcut-GC-MS and Needle Trap comprehensive GCxGC-TOF-MS for Breath Gas Analysis in the Clinical Environment. Anal Chem. 2010; 82(6):2541-51.

Filipiak W, Sponring A, Filipiak A, Ager C, Schubert J, Miekisch W, Amann A, Troppmair J.. TD-GC-MS analysis of volatile metabolites of human lung cancer and normal cells in vitro. Cancer Epidemiol Biomarkers Prev. 2010; 19(1): 182-95.

Miekisch W, Hengstenberg A, Kischkel S, Beckmann U, Mieth M, Schubert J.K. Construction and evaluation of a versatile CO2 controlled breath collection device. IEEE Sensors Journal. 2010; 10(1):211-215.

King J, Kupferthaler A, Unterkofler K, Koc H, Teschl S, Teschl G, Miekisch W, Schubert JK, Hinterhuber H, Amann A. Isoprene and acetone concentration profiles during exercise on an ergometer. J Breath Res. 2009; 3: 027006 (16pp).

Bajtarevic A, Ager C, Pienz M, Klieber M, Schwarz K, Ligor M, Ligor T, Filipiak W, Denz H, Fiegl M, Hilbe W, Weiss W, Lukas P, Jamnig H, Hackl M, Haidenberger A, Buszewski B, Miekisch W, Schubert J, Amann A. Noninvasive detection of lung cancer by analysis of exhaled breath. BMC Cancer. 2009; 9: 348.

Buszewski B, Wasiniak B, Ligor T, Nowaczyk J, Olszowy P, Miekisch W, Schubert JK, Amann A. Preparation and characterization of microporous fibers for sample preparation and LC-MS determination of drugs. J Sep Sci. 2009; 32(14):2448-54.

Mieth M, Kischkel S, Schubert JK, Hein D, Miekisch W. Multibed Needle Trap Devices for on Site Sampling and Preconcentration of Volatile Breath Biomarkers. Anal Chem. 2009; 81(14):5851-7.

Ligor M, Ligor T, Bajtarevic A, Ager C, Pienz M, Klieber M, Denz H, Fiegl M, Hilbe W, Weiss W, Lukas P, Jamnig H, Hackl M, Buszewski B, Miekisch W, Schubert J, Amann A. Determination of volatile organic compounds in exhaled breath of patients with lung cancer using solid phase microextraction and gas chromatography mass spectrometry. Clin Chem Lab Med. 2009; 47(5):550-60.

Schwarz K, Pizzini A, Arendack B, Zerlauth K, Filipiak W, Schmid A, Dzien A, Neuner S, Lechleitner M, Scholl-Bürgi S, Miekisch W, Schubert JK, Unterkofler K, Witkovský V, Gastl G, Amann A. Breath acetone - aspects of normal physiology related to age and gender as determined in a PTR-MS study. J. Breath Res. 2009; 3: 027003 (9pp).

Sponring A, Filipiak W, Mikoviny T, Ager C, Schubert J, Miekisch W, Amann A, Troppmair J. Release of volatile organic compounds from the lung cancer cell line NCI-H2087 in vitro. Anticancer Res. 2009; 29(1):419-26.

Filipiak W, Sponring A, Mikoviny T, Ager C, Schubert J, Miekisch W, Amann A, Troppmair J. Release of volatile organic compounds (VOCs) from the lung cancer cell line CALU-1 in vitro. Cancer Cell Int. 2008; 8(1):17.

Miekisch W, Kischkel S, Sawacki A, Liebau T, Mieth M, Schubert JK. Impact of sampling procedures onto results of breath analysis. Impact of sampling procedures on the results of breath analysis. J Breath Res. 2008; 2: 026007 (7pp).

Miekisch W, Fuchs P, Kamysek S, Neumann C, Schubert JK. Assessment of propofol concentrations in human breath and blood by means of HS-SPME-GC-MS. Clin Chim Acta. 2008; 395(1-2):32-37.

Kushch I, Arendacka B, Stolc S, Mochalski P, Filipiak W, Schwarz K, Schwentner L, Schmid A, Dzien A, Lechleitner M, Witkovsky V, Miekisch W, Schubert JK, Unterkofler K and Amann A. Breath isoprene – aspects of normal physiology related to age, gender and cholesterol profile as determined in a proton transfer reaction mass spectrometry study. Clin Chem Lab Med 2008; 46:1011-8.

Thelen S; Miekisch W; Halmer D; Schubert JK; Hering P; Muertz M. Inter-comparison of infrared cavity leak-out spectroscopy and gas chromatography /Flame ionization for trace analysis of ethane. Anal Chem 2008; 80:2768 – 2773.

Pabst F, Miekisch W, Fuchs P, Kischkel S, Schubert JK. Monitoring of oxidative and metabolic stress during cardiac surgery by means of breath biomarkers: an observational study. J Cardiothor Surg 2007; 2:37.

Schubert JK, Miekisch W, Fuchs P, Scherzer N, Lord H, Pawliszyn J, Mundkowski RG. Determination of antibiotic drug concentrations in circulating human blood by means of solid phase micro-extraction. Clinica Chimica Acta 2007; 386:57–62.

Birken T, Schubert JK, Miekisch W, Nöldge-Schomburg GFE. A novel visually CO2 controlled alveolar breath sampling technique. Tech Health Care 2006; 14(6):499-506.

Mueller SC. Jolanta Majcher-Peszynska J. Uehleke B, Klammt S. Ralf G. Mundkowski RG. Miekisch W. Sievers H. Bauer S, Frank B, Kundt G. Drewelow B. The extent of induction of CYP3A by St. John’s wort varies among products and is linked to hyperforin dose. Eur J Clin Pharmacol 2006; 62:29-36.

Schubert JK, Miekisch W, Birken T, Geiger K, Nöldge-Schomburg GFE. Impact of inspired substance concentrations onto results of breath analysis in mechanically ventilated patients. Biomarkers. 2005; 10:138-152.

Scholpp J, Schubert JK, Miekisch W, Noeldge-Schomburg GFE. Lipid peroxidation early after brain injury. J Neurotrauma. 2004; 21: 667-677.

Scholpp J, Schubert JK, Miekisch W, Geiger K. Breath markers and soluble lipid peroxidation markers in critically ill patients. Clin Chem Lab Med. 2002; 40:587-94.

Joarchi MS, Neiser U, Lenschow U, Schubert JK, Kienast W, Nöldge-Schomburg GFE, Steinhoff G. Thoratec left ventricular device for bridging to recovery in fulminant acute myocarditis. Ann Thorac Surg. 2002; 74:234-5.

Mols G, Hermle G, Schubert JK, Miekisch W, Benzing A, Geiger K. Volume-dependent compliance and VA/Q-mismatch in surfactant depleted isolated rabbit lungs. Crit Care Med. 2001; 29:144-51.

Schubert JK, Spittler K-H, Braun G, Geiger K, Guttmann J. CO2-controlled sampling of alveolar gas in mechanically ventilated patients. J Appl Physiol. 2001; 90:486-92.

Miekisch W, Schubert JK, Vagts D, Geiger K. Analysis of volatile disease markers in blood. Clin Chem. 2001; 47:1053-60.

Schubert JK, Esteban-Loos I, Geiger K, Guttmann J. In-vivo evaluation of a new method for chemical analysis of volatile components in the respiratory gas of mechanically ventilated patients. Technology and Healthcare. 1999; 7:29-37.

Müller WPE, Schubert JK, Benzing A, Geiger K. Method for the analysis of exhaled air by microwave energy desorption coupled with gas chromatography - flame ionisation detection – mass spectrometry. J Chromatogr B. 1998; 716:27-38.

Schubert JK, Müller WPE, Benzing A, Geiger K. Application of a new method for analysis of exhaled gas in critically ill patients. Intensive Care Med. 1998; 24:415-21.

Übersichtsartikel

Schubert JK, Miekisch W, Nöldge-Schomburg GFE. VOC breath markers in critically ill patients – potential and limitations. In: Breath analysis for clinical diagnostics and therapeutic monitoring. A Amann, D Smith Eds, World Scientific, London 2005, pp 267-92.

Schubert JK, Geiger K. Bedeutung und Perspektiven der Atemgasanalytik. Anästhesiol Intensivmed Notfallmed Schmerzther 1999, 34:391-95.

Schubert JK, Nöldge-Schomburg GFE. Die Pneumonie beim Transplantatempfänger: Diagnostik, Prognose und klinisches Procedere Transplantlinc 2000 2:3-11.

Miekisch W, Schubert JK, Noeldge-Schomburg GFE. Diagnostic Potential of Breath Analysis - Focus on Volatile Organic Compounds Clin Chim Acta 2004, 347:25-39.

Schubert JK, Miekisch W, Geiger K, Nöldge-Schomburg GFE. Breath analysis in critically ill patients - potential and limitations Expert Rev Mol Diagn 2004, 4(5):89-99.

Miekisch W, Schubert JK. From highly sophisticated analytical techniques to life-saving diagnostics: Technical developments in breath analysis TrAC Trends in Analytical Chemistry 2006, 25: 665-673.

Schubert JK, Miekisch W, Fuchs P, Nöldge-Schomburg GFE. Analytical sciences and application in medicine – Dr Jekyll and Mr Hyde? The science and education in Europe. Proceedings of the VIP meeting of Societas Humboldtiana Polonorum, Torun 9 – 12 March 2006; 160-167.

Schubert JK. Nanoparticles in medicine – friends or foes? Nanotechnology: Development and influence on the society (Societas Humboldtiana Polonorum) Conference Proceedings, Gdansk 24 – 26 May 2007; 157-171.

Miekisch W, Trefz P, Bergmann A, Schubert JK. Microextraction techniques in breath biomarker analysis. Bioanalysis. 2014 May;6(9):1275-91.

Buchbeiträge.

Miekisch W, Schubert JK, Müller WPE, Geiger K. Analysis of Exhaled Air as a New Means of Critical Care Testing. In: Advances in Critical Care Testing. WF List, MM Müller, MJ McQueen Eds, Springer 1999, 202-211.

Schubert JK, Miekisch W, Nöldge-Schomburg GFE. Volatile organic compounds as prognostic markers in ARDS. In: Disease Markers in Exhaled Breath. Basic Mechanisms and Clinical Applications. N Marczin, MH Yacoub Eds, IOS Press NATO Science Series, Amsterdam 2002, pp 338-43.

Schubert JK, Miekisch W, Geiger K. Exhaled breath markers in ARDS. In: Lung Biology in Health and Disease. Disease Markers in Exhaled Breath. N Marczin, S Kharitonov Eds, Marcel Dekker, New York 2003, 363-80.

Schubert JK. Pharmakologische Besonderheiten im Alter. In: Anästhesie 65 plus. A Benzing, T Loop Eds, Deutscher Ärzteverlag Köln 2009, 21-31.

Amann A, Miekisch W, Pleil J, Risby T, Schubert JK. Methodological issues of sample collection and analysis of exhaled breath. In: Exhaled Biomarkers. I Horvath, JC de Jongste Eds, European Respiratory Society Monograph, Vol. 49. 2010, 96-114.

Schubert JK, Miekisch W. Breath analysis in critically ill patients - potential and limitations. In: Volatile Biomarker. A Amann Ed., 2012.

Sukul P, Trefz P. Chapter 19: Physio-Metabolic Monitoring via Breath Employing Real-Time Mass Spectrometry: Importance, Challenges, Potentials, and Pitfalls. In: Bioanalytical Reviews, Ed. Springer Nature 2022 in press.

Miekisch W, Sukul P, Schubert J K. Chapter 2: Origin and Emission of Volatile Biomarkers in Breath: Basics and Dynamic Aspects. In: Volatile Biomarkers for Human Health. Ed. Haik H. The Royal Society of Chemistry 2022